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Low Temperature Storage and In Vitro Pollen Germination of Selected Spring Wheat Germplasms

Bahram Baninasab, Mirko Tabori, Junjie Yu, Yuxue Zhang, Xuelian Wang, Ian Deschiffart & Shahrokh Khanizadeh, 2017. Low Temperature Storage and In Vitro Pollen Germination of Selected Spring Wheat Germplasms, accepted: Journal of Agricultural Science. Vol. 9, No. 9; 2017

Abstract

The study was conducted to evaluate in vitro pollen germination of 50 spring wheat (Triticum aestivum L.) germplasms at three storage temperatures (23°C, 5°C, and -20°C). Germination of the mature pollen was measured right after harvest (0 time), and 24 h, 48 h, and 72 h after storage (HAS). Differences in fresh pollen germination between germplasms were significant and ranged from 21.15% to 1.09%. Pollen germination was the lowest at 24 h at room temperature. The pollens of 24 wheat germplasms (AAC Scotia, AW636, AW725, AW780, AW804, AW822, AW823, Brookfield, BRS Pardela, ECSW05, ECSW38, ECSW48, ECSW49, ECSW69, Hoffman, Kleos, Major, Nass, 12NQW-237, 12NQW-413, 12NQW-414, 12NQW-436, 12NQW-754, and 12BW0374) were viable after 24 h when they were stored at 5°C. Sub-zero temperature did not prolong pollen viability.

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