Deep sequencing leads to the identification of eukaryotic translation initiation factor 5a as a key element in Rsv1-mediated lethal systemic hypersensitive response to Soybean mosaic virus infection in soybean.

Chen, H., Arsovski, A.A., Yu, K., and Wang, A.M. (2016). "Deep sequencing leads to the identification of eukaryotic translation initiation factor 5a as a key element in Rsv1-mediated lethal systemic hypersensitive response to Soybean mosaic virus infection in soybean.", Molecular Plant Pathology. doi : 10.1111/mpp.12407  Access to full text

Abstract

Rsv1, a single dominant resistance locus in soybean, confers extreme resistance to the majority of Soybean mosaic virus (SMV) strains but is susceptible to the G7 strain. In Rsv1-genotype soybean, G7 infection provokes a lethal systemic hypersensitive response (LSHR), a delayed host defense response. The Rsv1-mediated LSHR signaling pathway remains largely unknown. In this study, we employed a genome-wide investigation to gain insight into the molecular interplay between SMV G7 and Rsv1-genotype soybean. sRNA-seq, degradome-seq and transcriptome-seq analyses were used to identify differentially expressed genes (DEGs) and miRNAs (DEMs) in response to G7 infection. A number of DEGs, DEMs and miRNA targets and the interaction network of DEMs and their target mRNAs responsive to G7 infection were identified. Knock-down of one of the identified DEGs, the eukaryotic translation initiation factor 5A (eIF5A), diminished the LSHR and enhanced viral accumulation, suggesting the essential role of eIF5A in the G7-induced, Rsv1-mediated LSHR signalling pathway. This work provides an in-depth genome-wide analysis of high-throughput sequencing data and identifies multiple genes and microRNA signatures that are associated with the Rsv1-mediated LSHR.

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