Characteristics of lingonberry plants propagated by in vitro and ex vitro propagation methods.

Debnath, S.C., Vyas, P., and Igamberdiev, A.U. (2012). "Characteristics of lingonberry plants propagated by in vitro and ex vitro propagation methods.", Acta Horticulturae (ISHS), 926, pp. 259-264.


Reproducible protocols for in vitro shoot proliferation from nodal explants and for the regeneration of adventitious shoots from leaf explants in lingonberry cultivars: ‘Regal’, ‘Splendor’ and ‘Erntedank’ have been standardized on a nutrient medium containing thidiazuron (TDZ) or zeatin. TDZ supported shoot proliferation and shoot regeneration at low (0.1 to 0.5 µM) and moderate (2-5 µM) concentrations, respectively but inhibited shoot elongation. TDZ initiated cultures were transferred to the medium containing 1-2 µM zeatin and produced usable shoots after one additional subculture. Such in vitro-derived tissue culture plantlets were grown ex vitro and compared with those propagated by conventional softwood cuttings, for growth, morphology and leaf antioxidant enzyme activity after four growth seasons. The tissue culture plants produced more stems, leaves, rhizomes and leaf antioxidant enzyme activities than the conventional cuttings. However, the increased vegetative growth of micropropagated plants did not persist when they were again propagated by softwood cuttings indicating that the juvenile phase induced by the in vitro culture did not persist in the second cycle of propagation.

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