Cloning and Analyzing of Cinnamyl Alcohol Dehydrogenase Gene from Pear (Pyrus) Fruit.
Tao, S., Zhao, M., Khanizadeh, S., and Zhang, S. (2012). "Cloning and Analyzing of Cinnamyl Alcohol Dehydrogenase Gene from Pear (Pyrus) Fruit.", South China Fruits, 41(3), pp. 40-45.
Cinnamyl alcohol dehydrogenase (CAD) is a key enzyme during lignin biosynthesizing. Two gene fragments coding for CAD designated as PB-CAD ( GenBank accession No.FJ478151) and PP-CAD ( GenBank accession No. FJ478152) were cloned and sequenced using RT-PCR from ‘Dangshansuli’ and ‘Kousui’ pear, respectively. Both of the two obtained CAD gene fragments are 689 bp, and they have only 4 different bases in nucleotide sequence, which was comprised of 229 residues, and the two identical amino acid sequences have a 99.6% identity to each other. Using sequence alignment, we found the pear amino acid sequence of the two CAD was 97.8% identical to CAD from Malus domestica （AAC06319）, 95% identical to CAD from Prunus mume （BAE48658）, 92.6% identical to CAD from Eriobotrya japonica （ABV44810） and 83.4% identical to CAD from Vitis vinifera （CAO21890）, respectively.
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